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coli cells were able to maintain a constant cytosolic pH of around 7.5 and the cell membrane was a chemically pure membrane that only allowed free diffusion of the uncoupler HAc: at equilibrium, the concentrations of HAc inside and outside the cell would be the same and the intracellular acetate concentrations would be approximately 3000 and 300 mM in media at pH 6.5 and pH 7.5, respectively